Journal: Journal of Cancer
Article Title: Eosinophil-Associated Genes are Potential Biomarkers for Hepatocellular Carcinoma Prognosis
doi: 10.7150/jca.95138
Figure Lengend Snippet: Multi-level experimental validation of differential gene expression in adjacent and HCC tissues. (A) Representative images of IHC validation showing the expression of the eosinophil marker gene EPX in adjacent non-tumor tissues and HCC samples. Black arrows indicate positive cells (B) Western blot analysis of the expression of EPX in adjacent and tumor tissues. (C) Differential expression of RAMP3, G6PD, SSRP1 and PLOD2 in adjacent and tumor tissues from the TCGA-LICN database. (D, E) Representative IHC images indicating the expression of RAMP3, G6PD, SSRP1 and PLOD2 in adjacent non-tumor tissues and tumor tissues. Scale bar represents 50 μm. (F) RT-qPCR showing the expression differences of RAMP3, G6PD, SSRP1 and PLOD2. *, P < 0.05; **, P < 0.01; and ***, P < 0.001.
Article Snippet: The primary antibodies are rabbit polyclonal antibodies against EPX, RAMP3 (bs-11972R, 1:2000, Bioss), G6PD (R26802, 1:1000, Zenbio), SSRP1 (ER1901-13, 1:1000, Huabio) and PLOD2 (bs-12731, 1:1000, Bioss).
Techniques: Expressing, Marker, Western Blot, Quantitative RT-PCR